A new platform for nucleic acid detection in biofluids

Reference Presenter Authors
André Pasa Pasa, A.(Universidade Federal de Santa Catarina); In this work is presented a platform for detecting nucleic acids. The platform consists of integrating a chemical-based approach for nucleic acid testing with a solid-state photomultiplier reader. We designed and synthesized a set of reagents that combined the chemical-based method with a chemiluminescent reaction. The signals generated were read out by the silicon photomultiplier. The platform sensitivity was determined by measuring known concentrations of a microRNA (hsa-miR-21-5p) with a limit of detection of 4.7 pmol/L. The platform was also used for detecting directly hsa-miR-21-5p in non-small cell lung cancer plasma samples. Circulating microRNAs have been identified as potential biomarkers for early detection, prognosis, and prediction of several diseases. An accurate device for microRNA profiling without extraction, pre-amplification, or pre-labeling of the target is now achievable and resulted in an innovative product (ODG platform) as diagnostic utility for the direct qualitative and quantitative analysis of microRNA biomarkers in biological fluids.
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